The secondary effluent of Berlin's sewage treatment plant Ruhleben was oxidized by dosages of 2.5-22 mg/L ozone and varying operation conditions to remove pharmaceutical compounds and disinfect water in parallel. The majority of analysed neutral and acidic drugs were efficiently removed to the detection limit at ozone consumptions equal to a dosage of 10-15 mg/LO3 for complete removal. A series of four iodinated organic contrast media (ICM) persisted the ozone treatment even at high consumption rates. Related to disinfection, the legal requirements (EU bathing water directive) could be fulfilled by a consumption of 10% could not be achieved by any treatment. The initial genotoxicity of the secondary effluent was stated by four independent tests. Due to the application of ozone, this genotoxicity was completely removed. The presented results confirm that ozonation can be a suitable advanced wastewater treatment at varying operation conditions to lower effluent concentrations of pharmaceuticals and active micro-organsisms.